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1968a,b). The success of these studies rests in good measure on the establishment of sufficient information concerning the thermal properties of relatively simple systems containing compounds important in biologic structures. , 1968c). ) and microscopic studies. Synthetic lipids are used in which the hydrocarbon chain structures as well as the polar groups are of one type. These well-defined compounds have characteristic and sharp transition temperatures which may be used as indicators to gain an insight into the inter-relationships of lipids in mixed systems.

0 - 70. Jf'- ______ Figure I. Langmuir-Wi Ihelmy surface balance. The Geiger tube for Ca 45 measurement is not shown, nor is the clean surface "Island" for Va measurement. J. GOERKE, H. H. HARPER, AND M. BOROWITZ 25 I iquid chromatography (GLC). 4°C, was obtained from Appl ied Science and had less than 1% "impurities by GLC. These I ipids were del ivered to the air-water surface in 20 to 30 ~g amounts from redisti I led Hexane solution using cal ibrated 30 to 50 ~I pipettes. 02 or as otherwise indicated using 2 mM Tris buffer (International Chemical and Nuclear Company) titrated with HCI or KOH.

A... E. Perlmann, J. BioI. , 242 (1967) 2739 lQ. J. Trurnit, J. , 15 (1960) 1. 11. B. Bull, Adv. , 3 (1947) 110. 12. P. Joos. Mededel Koninkl Vlaam. Acad. , 30 (1968) 3. 13. T. E. Alexander, J. , 27 (1968) 53. 14. T. B. , J. , 24 (1967) 310. 15. K. Hamaguchi, Bull. Chem. Soc. , 31 (1958) 123. 16. J. Mitchell, L. J. Palmer, Proc. 5th Intern. Congr. Surface Activity, Barcelona, 1968, in press. 17. R. J. Palmer, J. , 28 (1968) 168. 18. G. R. Ray, J. Phys. , 61, (1957) 1385. 19. F. Cheeseman and H.

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