By Miguel A Merchán, Javier De Felipe, Fernando de Castro
Cajal and De Castro's Neurohistological tools presents the 1st English translation of Fernando de Castro's 1933 e-book "Elementos de Técnica Micrográfica del Sistema Nervioso." A scholar of the famed founding father of glossy neuroscience, with Santiago Ramon y Cajal additionally serving because the Editor of the unique textual content, Fernando de Castro recorded all of the a number of protocols that were utilized in his laboratory via his scholars with the intention to supply a handbook of histological systems in particular designed for the effective constitution of the apprehensive process. This well known textual content is nearly unknown in its unique shape outdoor the Spanish-speaking world.
In a textual content that reads like a combination among a recipe ebook and an alchemical manuscript, authors Miguel Merchan, Javier DeFelipe, and Fernando de Castro (descendant of the 1933 publication's writer) placed the hot translation into old context. This ebook can also be superbly illustrated with plates of histological suggestions, offers a short consultant to new vocabulary, and the author's notes at the translated textual content. This pivotal paintings of vintage neurohistological concepts is a superb addition to the Cajal library.
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Additional info for Cajal and de Castro’s Neurohistological Methods
Cajal used this method in exocrine gland cells and proposed, for the first time, its role in cellular secretion (X 100). Original slides from Professor Jaime Merchán Cifuentes. Plate 19. Myelin staining. Del Rio Hortega’s method for staining ganglionar glia. Sympathetic ganglion from a cat (X5, X20). Photographs obtained from original slides from Professor Fernando de Castro (Archivo Fernando de Castro). Plate 20. Bolsi’s method using Cajal’s ammonium silver oxide (Ammonium bromide). Average staining quality.
He was determined to convince Cajal of the importance of the glial cells, which he saw as a diffuse endocrine gland that could play a part in diseases such as schizophrenia. Cajal then was working on the existence of a network described by Holmgren, which would connect the Golgi complex with the extracellular space, and he had invented a method using uranyl nitrate (136). This technique consistently stained 10 S E ction one : H istorical C ontext the Golgi apparatus, but also stained astrocytes, although rather sporadically.
Astrocytes and their “sucking feet” (end feet) attached to a blood vessel wall can be seen (X40). Original slides from Professor Jaime Merchán Cifuentes. Plate 21. Myelin staining procedures. Osmic acid. Top: low-power image of a peripheral nerve (X10). Bottom: magnification of nodes and paranodes, Schmidt-Lantermann’s incisures and spiny bracelet of Nageotte (top left corner X100). Photographs obtained from original slides from Professor Fernando de Castro (Archivo Fernando de Castro). Plate 22.