By Olov Lindberg, Lars Ernster
Chondria of tumours, for instance. are characterised through a low respiration strength. that could be linked to a poor mitochondrial association. because the physiological predicament of telephone respiratory appears to be like to lie within the formation of ATP. it's going to look that the criteria answerable for the splitting of ATP with the formation of phosphate esters. also are respon sible for the aforementioned keep watch over meChanism. The enzyme glucokinase, that is identified to be topic to hormonal keep watch over within the intact mobilephone constitution, is essentially sure to cytoplasmic debris. The activation procedures consist within the creation through the mitochondria of precursot.s to the components synthesized via the mobilephone. hence, except the enzymic spectrum invaluable for oxidative techniques and the formation of ATP. the mitochondria additionally include particular activation mechanisms, the character of that's made up our minds via the kind of cellphone during which they're con tained. Specialization could be saw within the sarcosomes of muscle groups, which serve to supply the contractile parts with A TP: within the mito chondria of kidney tubules, that are involved within the approaches of re absorption; in liver mitochondria, which concentrate on the construction of acetoacetate and within the synthesis of citrulline and detoxication conjugates of fragrant compounds: and never least within the mitochondria of embryonic tissue which. in line with the existing stag.e of improvement and the a part of the embryo within which they're positioned, play an energetic half within the differentiation approach.
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LHOCH, HAYAISHI and TEPLY 1952) whid1 catalyses the oxidation of DPNH2 by cytochrome c. It is a matter of doubt, however, whether this enzyme has the same activity in the isolated state as in the heart preparation (SLATER 1930 b). The long-known enzyme diaphorase, whic:h ·catalyses the oxidation of DPNH2 by methylene blue, but not by cytolhrome c, is thought to be an artefact (PoTTER 1950). Since the isolated DPNH2-cytochrome c reductase is not inhibited by antimycin a (EDELHOCH, HAYAISHI and TEPLY 1952), it would appear that this also is not an intact enzyme but the two end components of a three-component system, the central component, that carrying the factor, being lost in the preparation.
T nnd RuNNSTROM 1951. 1953). It mav also be of intncst to record prdiminary Yalucs obtained bY CLELA:Xll for the thcmical constitution of Yerscne mitoehondria. since these are. ). IERG and ERNSTER, Mitodwndria and Microsomes These experimental findings incline us to visualize a gradient of surface organization from the cyclophorase preparations of GREEN to the selectively permeable mitochondria, prepared in versene, at the highest level. At the cyclophorase level, this surface organization is responsible for a more or less intact osmotic system of a passive character; at the level of the intact mitochondrion, there is also an active enzymic organization which makes possible selective permeability and a metabolic regulation.
The metabolic cycle cannot be completed, since the condensation between the "active .. (\ body and glycine has not yet been elucidated. ln view of the fact that an ''active"' C 1 compound is said to he concerned here, it is conceivable that a solution of the matter· is to be sought among the reactions and possible co-factors that may be involved in the '"activation .. of the cl body. A possible association of this mechanism with the mitochondria is to be found in the faet that the co-factors ~which arc believed to be concerned, such as folic acid (TEPLY, d.