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By Shuang-Qing Zhang, Hong-Tao Jin, Feng Wei, Xiaohui Ma

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This Medicinal Materials DNA Barcode Database accepts all plastid DNA regions and nuclear ITS results for medicinal plant materials [28]. More recently, a universal andpublically available DNA barcoding system for identifying herbal materials has been established based on theITS2 and psbA–trnH barcodes (Figure 1). The system has been used for identifying herbal materials [29-32], and the methods have been approved for incorporation into supplement 3 of the Chinese Pharmacopoeia (2010 edition). During development of the DNA barcoding system as shown in Figure 1, voucher herbarium specimens of each species were verified based on their phenotypes.

2008;46(5):1771-1777. [49] XL. , Li Z, Du H, Xu X, Guo Z, Ma S, Li X, Lin R. Identification of raw Cuscutae Semen and its processed products by high performance liquid chromatography/diode-array detection/mass spectrometry (HPLC-DAD-MS) combined with principle component analysis. Journal of Liquid Chromatography and Related Technologies; 2014;37(5):748-759. [50] Chen Z, Liao L, Yang Y, Zhang Z, Wang Z. Different fingerprinting strategies to differentiate Porana sinensis and plants of Erycibe by high-performance liquid chromatography with diode array detection, ultra high performance liquid chromatography with tandem quadrupole mass spectrometry, and chemometrics.

Enough haplotypes of DNA sequences are required for the conservative evaluation of the restriction sites we select. As its result is not dependant on the PCR amplification, PCR-RFLP is an ideal method for Pharmacopeia with a better repeatability and less false possibility [8]. 2. Real-Time Quantitative PCR (RT-qPCR) PCR essentially any nucleic acid sequence present in a complex sample can be amplified in a cyclic process to generate a large number of identical copies that can readily be analyzed.

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