Download Rapid Diagnosis of Mycoplasmas by Itzahak Kahane, Amiram Adoni PDF

By Itzahak Kahane, Amiram Adoni

This compendium is the results of the FEMS Workshop on "Rapid analysis of Mycoplasmas" which I geared up and which happened in Jerusalem, Israel, August 11-23, 1991. the 1st week's classes have been held at a lodge at the outskirts of Jerusalem and consisted of lectures and discussions. This half used to be modelled alongside the strains of the Gordon convention within the united states, i.e., in an intimate atmo­ sphere during which all people may well combine and trade rules, and used to be very benefi­ cial. approximately a hundred scientists from all over the world attended the 1st week. Dur­ ing the 1st week, the biology, molecular biology and pathophysiology of myco­ plasmas, in addition to the entire major diagnostic equipment have been coated, together with either traditional and the more moderen applied sciences. The consultation on mycoplasmas within the human urogenital tracts used to be held together with the Israel Society for the learn and Prevention of Sexually Transmitted affliction. the second one week used to be a laboratory consultation and was once held on the Hebrew University-Hadassah scientific university campus in Ein Karem, Jerusalem. All ex­ periments have been performed by way of eminent experts of their box. The lab consultation had 36 individuals from 19 international locations who used the main glossy strategies for the prognosis of mycoplasmas in drugs, veterinary medication and agri­ tradition. The efficacy of numerous advertisement kits have been additionally established at the moment. i need to back thank everybody who helped and supported this paintings­ store, in addition to the authors of many of the chapters.

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Thomas, B. , Naidoo, O. and Taylor Robinson, D. (1993) The association of Mycoplasma genitalium with acute non-gonococcal urethritis. Lancet. (In press). , Gilchrist, C. and Taylor-Robinson, D. (1988) Infection of the epididymis by Ureaplasma urealyticum. Genitourin. Med. 64:367-368. Kass, E. -S. and McCormack, W. M. (1986) Low birth weight and maternal colonization with genital mycoplasmas. Pediatr. Infect. Dis. Suppl. 5:279-281. Katseni, V. , Gilroy, C. , Weber, J. N. and Taylor-Robinson, D. (1993) Mycoplasma fermentans in individuals seropositive and seronegative for HIV-l.

B. Gilroy and D. TaylorRobinson, unpublished data). , 1981). , 1989). , 1988) seem sufficient to incriminate the organisms and suggest that they may cause this disease occasionally. Although ureaplasmas have been associated with altered sperm motility, specimens from fertile and infertile subjects contain the organisms with similar frequency and the results of antibiotic trials are not in favour of ureaplasmas alone causing male infertility (Taylor-Robinson, 1986). In view of the role of ureaplasmas in NGU, it would not be surprising to find that they were involved in sexually acquired reactive arthritis (SARA).

Depending on the number of organisms present in the clinical specimens, a positive result could be obtained as early as 48 h after inoculation of the cover-slip chamber (E. Jacobs, Freiburg, personal communication). This is of great advantage as compared to the culture procedure, which reveals visible colonies not before 10 to 21 days after inoculation of the specimen onto agar plates. , gene probes, polymerase chain reaction (peR) or immunological methods (capture-ELISA) become available, the cover-slip technique is a rather rapid, yet simple method for identification of M.

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