By Andrea Borini, Giovanni Coticchio
Oocyte cryopreservation involves very important capability benefits for humanIVF, providing a much less ethically disputable replacement to embryo cryopreservation,simplifying and making more secure oocyte donation, and giving a chance forfertility maintenance to girls prone to untimely ovarian failure as an effectof genetic components or chemo- or radiotherapies. Oocyte cryopreservation couldalso meet the expectancies of girls wishing to maintain their fertility forsocial purposes. within the previous few years, advances in cryopreservationmethodologies have dramatically more suitable the potency of oocytecryopreservation, resulting in the beginning of over 1000 infants andchallenging the supremacy of embryo cryopreservation because the preferredform of fertility upkeep. this article has been conceived with the purpose of providing a finished view of the cutting-edge of oocyte cryopreservation. It covers basic thoughts of low temperature garage (controlled fee gradual cooling andvitrification), points of oocyte body structure correct to the method ofcryopreservation, crucial organic and scientific proof, and ethicalimplications of oocyte cryopreservation, thereby supplying a completeoverview of development during this technique in assisted copy.
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Oocyte cryopreservation involves vital power benefits for humanIVF, providing a much less ethically disputable replacement to embryo cryopreservation,simplifying and making more secure oocyte donation, and giving a chance forfertility protection to girls susceptible to untimely ovarian failure as an effectof genetic elements or chemo- or radiotherapies.
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Extra resources for Preservation of Human Oocytes (Reproductive Medicine and Assisted Reproductive Techniques)
Theriogenology 2006; 66: 1026–33. A HISTORICAL OVERVIEW OF OOCYTE AND EMBRYO PRESERVATION 104. 105. 106. 107. 108. 109. 110. 111. 112. 113. 114. 11 Gook DA, Osborn SM, Bourne H, et al. Fertilization of human oocytes following cryopreservation; normal karyotypes and absence of stray chromosomes. Hum Reprod 1994; 9: 684–91. Chung HM, Hong SW, Lim JM, et al. In vitro blastocyst formation of human oocytes obtained from unstimulated and stimulated cycles after vitriﬁcation at various maturational stages.
27. 28. 29. 30. 31. 32. 33. K. K. 2 INTRODUCTION Storage of unfertilized mammalian oocytes has numerous applications in both human and animal reproductive technologies. Cryopreserved human oocytes can assist in the treatment of human infertility, for example, where loss of fertility may be predicted following ablative anticancer treatments or in patients suffering premature ovarian failure; banking of excess oocytes produced as a result of in vitro fertilization (IVF) treatments; and donation of oocytes to others (storage giving the added advantage of allowing time to screen donors for disease).
J Gen Physiol 1963; 47: 347–69. Leibo SP. Fundamental cryobiology of mouse ova and embryos. In: the freezing of mammalian embryos. Ciba Foundation Symposium 52 (new series) Amsterdam: Elsevier, 1977: 69–92. Luyet BJ, Gehenio PM. Life and death at low temperatures. Normandy, MO: Biodynamica, 1940. Sutton RL. Critical cooling rates to avoid ice crystallization in solutions of cryoprotective agents. J Chem Soc Faraday Trans 1991; 87: 101–5. Fahy GM, MacFarlane DR, Angel CA, Meryman HT. Vitriﬁcation as an approach to cryopreservation.